SnB Parameters

SnB Parameters

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Notes:

PH8755: is a 9-residue peptide antibiotic

having a pure alpha-helical conformation

contains 5 residues of the unusual amino acid

alpha-aminoisobutyric acid

ToxII: Scorpion Protein Toxin

64 residue scorpion toxin

0.96A data

only information known: approx 500 atoms & 4 disulfide bonds

starting model: 5 fragments varying in length from 5 to 18 residues for a total of 241 atoms

structure revealed following Xplor refinement and Fourier maps

60/64 residues identified correctly

final R factor of 0.160 for

512 protein atoms,

112 water molecules and

418 protein hydrogen atoms

Phase error of 19 deg. between SnB phases and final