Welcome

SMARTEn (System for Mobile Analysis in Real-Time of ENvironment)

SMARTEn aims to provide new ways to analyze metagenomic DNA using fully mobile setup.

Our Hardware

To run the project we are using two key pieces of technology. The first one is MinION sequencer from Oxford Nanopore Technology. The pictures below show one of the three devices we are using in our labs.

MinION MinION

The second component is a Supercomputer On a Chip (SCoC). The specific platform we are using is based on NVIDIA’s Jetson Nano. Below we provide pictures of the entire setup. We include also specification if you are interested in building a similar box for your project (please note that we are not endorsing any particular provider).

MinION MinION MinION

SCoC Harware Specification

Software Specification

Jetson Nano comes with its own variant of Ubuntu Linux for Tegra – the specific version is Ubuntu 18.04.4 LTS (GNU/Linux 4.9.140-tegra aarch64). Since this is fully functional Linux, we have access to all common tools, including compilers, like gcc and clang, and the entire NVIDIA CUDA development environment (currently we are using CUDA 10.0).

Of course, the key requirement is to be able to run Oxford Nanopore tools. ONT provides MinKNOW framework in its repository. Just running fairly standard chain of Debian/Ubuntu apt commands brings in all tools (notice that we are using xenial repository):

echo "deb http://mirror.oxfordnanoportal.com/apt xenial-stable-minit non-free" > /etc/apt/sources.list.d/nanoporetech.list

apt update && apt upgrade

apt install minknow-core-minit-offline ont-bream4-minit ont-configuration-customer-minit ont-minknow-frontend-minit ont-minknow-static-frontend

The only caveat is that currently, it is not possible to install guppy basecaller directly from packages (there are several reasons: first, dependencies that cannot be satisfied without downgrading Ubuntu, second, lack of support for NVIDIA SM53 in the default package). However, we were able to easily bypass these problems by compiling (with a bit of editing) guppy from sources. Note that in order to get access to the sourcecode you have to joint ONT developers program. Below is a glimpse at MinKNOW running on NVIDIA Nano.

MinION

We will be sharing our experiences and updating this web page as the project progresses!

References